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| Registros recuperados : 7 | |
| 3. |  | CHUEIRE, L. M. de O.; MARIANOWSKI, T.; CAMPO, R. J.; HUNGRIA, M. Beneficios obtidos pela inoculação do feijoeiro com estirpes de Rhizobium eficientes e competitivas. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 25.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 9.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 7.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 4., 2002, Rio de Janeiro. Fertbio 2002: agricultura: bases ecológicas para o desenvolvimento social e econômico sustentado. Rio de Janeiro, 2002. p. 172. Guia do congressista e em CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 4. | | LOUREIRO, M, de F.; MARIANOWSKI, T.; NICOLÁS, M. F.; ELIAS NETO, N.; HUNGRIA, M. Uso de marcadores moleculares para la identificación de especies de Discolobium nativas del Pantanal Matogrosense de Brasil. In: REUNIÓN LATINOAMERICANA DE RHIZOBIOLOGIA, 21.; CONGRESO NACIONAL DE LA FIJACIÓN BIOLÓGICA DE NITRÓGENO, 6., 2002. Cocoyoc. Memorias... [S.l.]: Centro de Investigación sobre Fijación de Nitrógeno; Universidade Nacional Autónoma de México, 2002. p. 91.| Biblioteca(s): Embrapa Soja. |
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| 6. | | ELIAS NETO, N.; LOUREIRO, M. de F.; NICOLÁS, M. F.; MARIANOWSKI, T.; TORRES, A. R.; HUNGRIA, M. Identification of Discolobium species indigenous to the Brazilian Pantanal ecosystem by microsatellite (SSRs) markers. Semina, Ciência Agrárias, Londrina, v. 33, Supl. 1, p. 3017-3022, 2012.| Biblioteca(s): Embrapa Soja. |
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| 7. | | NICOLÁS, M. F.; HUNGRIA, M.; ARIAS, C. A. A.; MARIANOWSKI, T.; CAMPO, R. J.; NODARI, R, O. Desempenho simbiótico de cultivares de soja e busca de marcadores moleculares relacionados à fixação biológica do nitrogênio (04.2001.338-2). In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2002: microbiologia de solos. Londrina: Embrapa Soja, 2003. p. 59-75. (Embrapa Soja. Documentos, 216).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 7 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
07/10/2020 |
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Data da última atualização: |
09/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MALOSSI, C. D.; FIORATTI, E. G.; CARDOSO, J. F.; MAGRO, A. J.; KROON, E. G.; AGUIAR, D. M. de; BORGES, A. M. C. M; NOGUEIRA, M. F.; ULLMANN, L. S.; ARAUJO JUNIOR, J. P. |
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Afiliação: |
CAMILA DANTAS MALOSSI, São Paulo State University (Unesp), Botucatu; EDUARDO GORZONI FIORATTI, Vales do Jequitinhonha e Mucuri Federal University (UFVJM); JEDSON FERREIRA CARDOSO, Evandro Chagas Institute, Ananindeua; ANGELO JOSE MAGRO, São Paulo State University, Unesp, Botucatu; ERNA GEESSIEN KROON, Universidade Federal de Minas Gerais; DANIEL MOURA DE AGUIAR, Mato Grosso Federal University, Cuiabá; ALICE MAMEDE COSTA MARQUE BORGES, Mato Grosso Federal University, Cuiabá; MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP; LEILA SABRINA ULLMANN, São Paulo State University, Unesp; JOÃO PESSOA ARAUJO JUNIOR, São Paulo State University, Unesp. |
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Título: |
High genomic variability in Equine Infectious Anemia Virus obtained from naturally infected horses in Pantanal, Brazil: an endemic region case. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Viruses, v. 12, n. 2, 207, p. 1-15, 2020. |
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DOI: |
10.3390/v12020207 |
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Idioma: |
Português |
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Conteúdo: |
Equine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectiousanemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory inthis area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAVare currently available only forgagand LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic areain Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing(NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning,Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilians train comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions. MenosEquine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectiousanemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory inthis area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAVare currently available only forgagand LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic areain Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing(NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning,Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilians train comprises a separate monophyletic group. Th... Mostrar Tudo |
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Thesagro: |
Anemia Infecciosa; Doença Animal; Eqüino; Vírus. |
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Thesaurus NAL: |
Endemic diseases; Equine infectious anemia virus. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216482/1/HighGenomicVariability-2020.pdf
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Marc: |
LEADER 02584naa a2200313 a 4500
001 2125345
005 2020-10-09
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3390/v12020207$2DOI
100 1 $aMALOSSI, C. D.
245 $aHigh genomic variability in Equine Infectious Anemia Virus obtained from naturally infected horses in Pantanal, Brazil$ban endemic region case.$h[electronic resource]
260 $c2020
520 $aEquine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectiousanemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory inthis area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAVare currently available only forgagand LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic areain Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing(NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning,Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilians train comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions.
650 $aEndemic diseases
650 $aEquine infectious anemia virus
650 $aAnemia Infecciosa
650 $aDoença Animal
650 $aEqüino
650 $aVírus
700 1 $aFIORATTI, E. G.
700 1 $aCARDOSO, J. F.
700 1 $aMAGRO, A. J.
700 1 $aKROON, E. G.
700 1 $aAGUIAR, D. M. de
700 1 $aBORGES, A. M. C. M
700 1 $aNOGUEIRA, M. F.
700 1 $aULLMANN, L. S.
700 1 $aARAUJO JUNIOR, J. P.
773 $tViruses$gv. 12, n. 2, 207, p. 1-15, 2020.
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Embrapa Pantanal (CPAP) |
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